General Cell Based Assays
Eurofins Discoverx InCELL Hunter eXpress BLK Kinase Binding Assay, 10pl Kit. (96-0044E1CP0L)
The InCELL Hunter eXpress BLK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BLK via detection of BLK levels. This product uses EFC technology. The cell line included overexpresses BLK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF HMGB1 Detection Kit, 500 Assay Points
HTRF HMGB1 Detection Kit, 500 Assay Points
Eurofins Discoverx cAMP Hunter™ Rat DRD1 Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Rat DRD1 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure rat DRD1 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human CD27 Signaling Reporter Cell-Based Assay Kit (HEK 293)
The PathHunter Human CD27 Signaling NF-kB Reporter Stable Cell Line Assay (HEK 293) measures NF-kB pathway activation through detection of induced expression levels of a tagged reporter protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ABclonal Technology HRH2 Rabbit pAb
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Eurofins Discoverx PathHunter eXpress C3AR1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0814E3CP0L)
The PathHunter C3AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C3a) based activation of C3AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C3AR1 and EA-tagged β-Arrestin-2. Activation of C3AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress MTNR1A CHO-K1 GPCR Assay, 2-Plate Kit. (95-0086E2CP2M)
The cAMP Hunter MTNR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melatonin) based activation of MTNR1A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MTNR1A, a naturally Gi/Go coupled GPCR. Upon activation of MTNR1A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0083E2CP2M)
The cAMP Hunter DRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD1, a naturally Gs coupled GPCR. Upon activation of DRD1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress A549 GLP Methyltransferase Assay, 10-plate Kit. (96-0045E15CP7L)
The InCELL Hunter eXpress A549 GLP Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of GLP via detection of GLP levels. This product uses EFC technology. The cell line included overexpresses GLP target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGER3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0640E2CP0M)
The PathHunter PTGER3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER3 and EA-tagged β-Arrestin-2. Activation of PTGER3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress OXER1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0089E2CP2L)
The cAMP Hunter OXER1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 5-oxo-ETE) based activation of OXER1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OXER1, a naturally Gi/Go coupled GPCR. Upon activation of OXER1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TGFBR1/ACVR2B Dimerization Assay, 2pl Kit. (93-1046E3CP5M)
The PathHunter TGFBR1/ACVR2B Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF-11) induced dimerization of TGFBR1/ACVR2B receptors. This product uses EFC technology. The cell line included overexpresses ACVR2B target protein fused to PK alongside TGFBR1 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/ACVR2B, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TRHR Total Internalization Assay, 10pl Kit. (93-0949E3CP5L)
The PathHunter TRHR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of TRHR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged TRHR and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the TRHR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mRXFP3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0928E2MCP2M)
The PathHunter mRXFP3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of mRXFP3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mRXFP3 and EA-tagged β-Arrestin-2. Activation of mRXFP3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mRXFP4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0929E2MCP2L)
The PathHunter mRXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. insulin-like 5, relaxin-3) based activation of mRXFP4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mRXFP4 and EA-tagged β-Arrestin-2. Activation of mRXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.